Results in Context

Equine ICSI Success Rates Explained

Understand maturation, cleavage, blastocyst, embryos-per-mare, pregnancy, and live-foal results without mistaking one stage for another.

Written by Solo Select Horses Reviewed July 16, 2026

Equine laboratory room at Solo Select

The short answer

There is no single equine ICSI success rate. Results are measured at several stages: oocyte recovery, maturation, cleavage after injection, blastocyst development, pregnancy after transfer, and ultimately live foals. A percentage is meaningful only when its denominator, sample, reporting period, and endpoint are stated.

01

The ICSI results funnel

Begin with follicles aspirated, then follow oocytes recovered, mature oocytes, injected oocytes, cleaved embryos, blastocysts, embryos transferred, pregnancies, and live foals. Attrition can occur at every transition.

A laboratory may calculate blastocyst rate from all recovered oocytes, mature injected oocytes, or cleaved embryos. Those percentages are not directly interchangeable. The published denominator must travel with the number whenever it is repeated.

02

GeneTech's published operating figures

GeneTech currently publishes the following results on its website: 60.3% maturation, 73.6% cleavage, 35.3% blastocyst rate among cleaved embryos, 1.67 average blastocysts per mare, and 67.3% pregnancy rate. GeneTech also states that it produced more than 6,000 embryos during the 2022 and 2023 breeding seasons.

These are GeneTech-reported operating figures, not a forecast for an individual mare. The public summary does not state the complete sample size, reporting period for every rate, pregnancy-check day, fresh-versus-frozen transfer mix, or whether “per mare” means per aspiration session. Breeders should request the current definitions and dataset when comparing laboratories.

  • Maturation: 60.3%
  • Cleavage: 73.6%
  • Blastocyst rate among cleaved embryos: 35.3%
  • Average blastocysts per mare: 1.67
  • Pregnancy rate: 67.3%
  • More than 6,000 embryos reported for the 2022–2023 breeding seasons

03

Questions to ask about any percentage

Ask what was counted, what it was divided by, how many cases were included, when the work occurred, and whether shipped and in-house oocytes were combined. For pregnancy data, ask the day of examination, embryo type, warming status, recipient source, and whether losses after the first positive examination are included.

A current live-foal rate is the most complete outcome but takes the longest to mature and may reflect transfer and pregnancy management outside the ICSI laboratory. Embryo-production data and foal-production data therefore measure related but different systems.

  • What is the numerator?
  • What is the denominator?
  • How large is the sample?
  • What dates does it cover?
  • Is the result per oocyte, aspiration, embryo, transfer, or mare?
  • Are fresh and vitrified embryos separated?
  • At what pregnancy day was success recorded?

04

Why an individual mare can differ

Mare age, follicle population, oocyte competence, reproductive history, semen quality, handling, laboratory conditions, embryo development, recipient timing, and simple biological variation can all affect the path from aspiration to foal.

An average describes a group. It does not promise that a particular aspiration will produce the average number of oocytes or embryos. Zero embryos and multiple embryos are both possible outcomes from an individual session.

Common Questions

Direct answers

What is a good blastocyst rate for equine ICSI?
A blastocyst percentage cannot be evaluated responsibly without its denominator and sample. Ask whether it is calculated from recovered oocytes, mature injected oocytes, or cleaved embryos, then compare like with like.
Does a cleavage rate equal an embryo rate?
No. Cleavage is an early developmental milestone after injection. Not every cleaved embryo reaches the transferable or freezable blastocyst stage.
Does an embryo guarantee a pregnancy?
No. A transferable embryo must still survive handling or warming, be transferred into an appropriate recipient, establish pregnancy, and continue developing.

Sources and Methodology

Where these answers come from

GeneTech-specific statistics are identified as partner-reported operating figures. General medical and biological explanations use veterinary or primary technical sources. Percentages should not be compared unless their denominator, population, endpoint, and reporting period match.

From follicle to embryo

ICSI is a sequence,
not one success rate

Every stage has its own scientific name, biological checkpoint, and denominator. Reaching one stage does not guarantee the next.

  1. Stage 01Collection day

    Transvaginal follicular aspiration

    Oocyte pickup (OPU)

    A veterinarian uses ultrasound guidance to aspirate follicles from the sedated donor mare and recover cumulus–oocyte complexes.

  2. Stage 02Laboratory stage

    In vitro maturation to metaphase II

    The oocytes mature

    Recovered oocytes are cultured and evaluated. Only oocytes that reach the mature MII stage are candidates for sperm injection.

  3. Stage 03ICSI

    Intracytoplasmic sperm injection

    One sperm is injected

    An embryologist selects a sperm cell and places it directly inside each mature oocyte. Injection begins development; it does not guarantee an embryo.

  4. Stage 04Early culture

    Embryonic cleavage

    Cells begin dividing

    A successfully activated oocyte may divide from two cells to four, eight, and beyond. Cleavage is an early milestone—not the final embryo rate.

  5. Stage 05Mid-culture

    Compaction and morula formation

    The cells organize

    The dividing cells compact into a morula. Some cleaved embryos stop before this stage, which is why cleavage and blastocyst rates differ.

  6. Stage 06Commonly D7–D8

    Blastocyst development

    A transferable embryo forms

    An embryo that reaches the blastocyst stage may be evaluated for fresh transfer or vitrification. Transfer, pregnancy, and live foal remain later outcomes.

The embryo is not the finish line. Warming or fresh handling, recipient transfer, pregnancy establishment, ongoing gestation, and live foal are separate later outcomes.

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